Device

Part:BBa_K360122:Design

Designed by: Claudia Ivonne Hernandez Armenta   Group: iGEM10_UNAM-Genomics_Mexico   (2010-10-21)


Promoter + RBS + Lumazine + terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 395
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We haven't characterized t yet, so compared with other spacers we don't know the exact yield.


Source

The seven bases spacer is the one from Escherichia coli's LacZ gene.

References